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. 2014 Feb 25;9(2):e90187. doi: 10.1371/journal.pone.0090187

Figure 3. Microtubules, but not actin filaments, are required for intracellular movement of endocytosed AChR vesicles.

Figure 3

A, Representative images showing the close association of BBQ crosslinking-induced AChR vesicles (BBQ) and microtubule structures (tubulin) in cultured muscle cells (arrows). The integrity of the microtubule network was effectively perturbed by treatment with nocodazole (Noc) or exposure to 4°C, which led to the fragmentation of the microtubule structures; however, microtubules were not affected by latrunculin A (Ltn A) treatment. Crosslinking-induced AChR endosomes were detected but were not associated with microtubule (arrowheads). The boxed regions were magnified by 8 times for clarity. B, Quantification showing the differential effects of cytoskeletal disruption of microtubules and actin filaments on the movement of crosslinking-induced AChR vesicles. Data are mean ± SEM; n = 30; *p<0.001 (Student’s t test).