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. 2014 Feb;33(2):115–122. doi: 10.5732/cjc.012.10236

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Chinese Journal of Cancer

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License, which allows readers to alter, transform, or build upon the article and then distribute the resulting work under the same or similar license to this one. The work must be attributed back to the original author and commercial use is not permitted without specific permission.

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Figure 1. — A, GSCs grow as neurosphere-like gliomaspheres in stem cell medium (original magnification × 20). B, GSCs adhered to poly-lysine-coated plates when cultured in 10% FBS/DMEM/F12 medium (original magnification × 100). C, the expression of neuronal class III beta-tubulin-1 (TUJ-1), a neuronal marker on the GSC sphere (original magnification × 200). D, the expression of glial fibrillary acidic protein (GFAP), an astrocytic marker in the GSC sphere (original magnification × 200). E-G, immunostaining of tumor cells for neural stem cell markers, Nestin (E), CD133 (F), and Sox-2 (G), in the GSC sphere (original magnification × 200).