Table 4.
Mutations in the CYP51A gene of A. fumigatus detected directly from BAL fluid samples
| No. of samples | Amino acid substitutions caused by non-synonymous mutations | Polymorphisms caused by synonymous mutations | Culture/azole susceptibility |
|---|---|---|---|
| Samples carrying single or multiple non-synonymous mutations | |||
| 1 | N425S | A. fumigatus/susceptible | |
| 1 | M172Va, F46Ya | A. fumigatus/susceptible | |
| 1 | L206P, L210P | G89G | negative/NA |
| 1 | K67E | G89G | Penicillium species/POS = 1, VRC = 4, ITC > 4 |
| 1 | Y107C | D70D, G89G | negative/NA |
| 1 | M220Vb | G89G | A. fumigatus/susceptible |
| 1 | N33D, K80E | G89G | ND/NA |
| 1 | F41S, E66G | D70D, G89G | ND/NA |
| 1 | P216Lc | G89G | negative/NA |
| Samples carrying synonymous mutations only | |||
| 1 | G89G, F495F | ND/NA | |
| 4 | D70D, G89G | 3 ND; 1 negative/NA | |
| 1 | V44V, G89G | negative/NA | |
| 1 | G89G, F165F | A. fumigatus/susceptible | |
| 38 | G89G | one resistant A. fumigatus isolate; POS = 1, VRC = 2, ITC > 4 | |
NA, not available; ND, culture not done; ITC, itraconazole; POS, posaconazole; VRC, voriconazole.
bMutation confirmed to be associated with azole resistance in A. fumigatus;40 shown in bold due to its confirmed association with azole resistance in A. fumigatus.
cMutation confirmed to be associated with azole resistance in A. fumigatus;15 shown in bold due to its confirmed association with azole resistance in A. fumigatus.