Figure 1.
ASIC1a channels are present on BLA interneurons. a, Typical linear currents (Ih is absent) recorded from presumed interneurons in response to 1 s long hyperpolarizing voltage steps (10 mV increments from the holding potential of −70 mV) in voltage-clamp (V-clamp) mode (left), and an example of fast, nonaccommodating spiking of interneurons in response to 500 ms long current injections in the current-clamp (C-clamp) mode (right). b, Brief (200 ms) pressure application of acidified solution (left) or 40 mm ammonium (right) induced inward currents in interneurons, which were increased by lowering the bath temperature. c, Currents evoked by acidified solution or 40 mm ammonium were blocked by bath-applied flurbiprofen (2 mm). d, In the current-clamp mode, bath application of 5 mm ammonium-induced high-frequency firing. In b and c, Vh is −70 mV. In b, c, and d, recordings are in the presence of CNQX (10 μm), d-AP5 (50 μm), bicuculline (20 μm), and SCH50911 (10 μm).