. 2014 Mar;42(3):334–342. doi: 10.1124/dmd.113.054726

TABLE 1.

T-5 N-oxide kinetic constants

Kinetic constants were determined using 10 pmol/ml recombinant P450 or 0.1 mg/ml HLM and are reported as pmol/min/pmol P450 except with pooled HLM where the units are pmol/min/mg microsomal protein.

Enzyme	K_m	V_max	V_max/K_m
	µM	pmol/min/pmol
rCYP2C8	1.6	.22	0.14
rCYP2D6	31	.30	0.01
rCYP3A4	6.4	.06	0.01
rCYP3A5	2.6	13.9	5.4
HLM	5.1	19.6^a

^{^a}

Units are pmol/min/mg microsomal protein.