Fig. 4.

5′-RACE analysis of SULT1C3 mRNA in LS180 cells. 5′-RACE was performed using a SULT1C3 gene-specific primer located in exon 6 (A). The PCR products were briefly run, visualized (A) and recovered from an agarose gel. The products were then ligated into the pGEM-T Easy plasmid, and 5 clones were sequenced (B). The sequence of the SULT1C3 gene beginning just upstream of a computationally predicted TATA box (TTATA) and ending just downstream of the predicted translation start codon in exon 2 (ATG) is shown aligned with the corresponding regions of the 5′-RACE clones. The approximate transcription start site is at nt 108,866,427 of NC_000002.11 (A), the end of exon 1 is at nt 108,866,539 (G), and the translation start site is at nt 108,873,651. The position of a nucleotide difference that was consistently detected in the 5′-RACE clones relative to the corresponding gene sequence is marked with an asterisk.