Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Feb 26;5:61. doi: 10.3389/fmicb.2014.00061

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2014 Lee, Burow, Woebken, Everroad, Kubo, Spormann, Weber, Pett-Ridge, Bebout and Hoehler.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Paired Chlorophyll a natural fluorescence, confocal, CARD-FISH, and ¹³C-acetate NanoSIMS (permil) micrographs of GN-S mat (A–H). Chloroflexi (CFX1223 and GNSB-941) (A–D) and Desulfosarcina/Desulfobacteraceae (DSS658) hybridized filaments (E–H) shown in conjunction with Chlorophyll a natural fluorescence, CARD-FISH, ¹³C enrichment, and secondary electron images, respectively. Bar represents 2 μm. Quartile box plots of ¹³C-acetate NanoSIMS spot measurements from Elkhorn Slough (ES), GN-S, and GN-I mats shown in (I) with significant difference test comparison to isotopic spot measurements on microbial filaments showing no hybridization to either probe. Asterisk denotes outliers 1.5^*IQR outside quartiles (though still kept for analysis). “#” denotes both Shapiro–Wilk test p < 0.05 and Wilcoxon difference test p < 0.05 (compared to unhybridized filaments). “N.D.” indicates no hybridized filaments were detected. ES denotes Elkhorn Slough mat samples, CF denotes CFX1223 and GNSB-941 probes, and DSS denotes DSS658 probes.