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. 2014 Mar;85(3):408–419. doi: 10.1124/mol.113.090043

Fig. 1.

Fig. 1.

The PDE5 inhibitor sildenafil interact with established cytotoxic chemotherapy agents to kill multiple bladder cancer cell lines. (A) Bladder cancer cells (HT-1376; J82; T24) were treated with mitomycin C (MITO 100–200 nM) and/or sildenafil (SIL, 2.0 μM). Cells were isolated after 24 hours, and viability was determined by trypan blue exclusion (n = 3, ± S.E.M.). #P < 0.05 greater than corresponding value in vehicle (VEH) control. (B) Bladder cancer cells (HT-1376; J82; T24) were treated with DOX (200–400 nM) and/or SIL (2.0 μM). Cells were isolated after 24 hours, and viability was determined by trypan blue exclusion (n = 3, ± S.E.M.). #P < 0.05 greater than corresponding value in vehicle control. (C) Bladder cancer cells (HT-1376; J82; T24) were treated with cisplatin [cisplatinum (CDDP); 1000–2000 nM] and/or SIL (2.0 μM). Cells were isolated after 24 hours, and viability was determined by trypan blue exclusion (n = 3, ± S.E.M.). #P < 0.05 greater than corresponding value in vehicle control. (D) Bladder cancer cells (HT-1376; J82; T24) were treated with Gemzar (25–50 nM) and/or SIL (2.0 μM). Cells were isolated after 24 hours, and viability was determined by trypan blue exclusion (n = 3, ± S.E.M.). #P < 0.05 greater than corresponding value in vehicle control. (E) Bladder and pancreatic cancer cells (T24, PANC-1, Mia Paca2, AsPC-1) were treated with Gemzar (25 nM) and/or paclitaxel (TAX, 10 nM) and/or SIL (2.0 μM). Cells were isolated after 24 hours, and viability was determined by trypan blue exclusion (n = 3, ± S.E.M.). #P < 0.05 greater than corresponding value in vehicle control.