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. 2014 Feb 26;5:21. doi: 10.3389/fendo.2014.00021

Figure 3.

Figure 3

The influence of different culture media on cell survival and expression of genes related to germ cell differentiation in a three-dimensional cultures of rat testicular cells. (A) The cells were cultured for 7 days in DMEM + glutamine (GL), DMEM + Glutamax (GLMAX), or F12 (presented on the X-axis) either with (light columns) or without (dark columns) hCG and rFSH stimulation. The percentage of apoptotic (TUNEL-positive) cells, normalized to the 1-day value, is shown on the Y-axis. (B–E) The cells were cultured for 0, 7, and 21 days. The graphs depict the relative expression of rat Zbtb16 (also known as Plzf) (B), Kit (C), Dazl (D), and Crem (E) (determined by qPCR analysis with Actb as an internal control) by cells cultured in DMEM + glutamine (DMEM + GL), DMEM + Glutamax (DMEM + GLMAX), or F12. On the X-axis, the different periods of culture [0 (D0), 7 (D7), and 21 (D21) days] are depicted and the Y-axis shows the mean relative expression of replicates calculated by the ddCt procedure. Student’s t-test was applied to compare the different experimental conditions. *p < 0.05, **p < 0.01, ***p < 0.001.