Fig. 4.

Effect of TLR3/8 activation on IRF expression. Human neuronal cells (primary neurons, NT2-N cells, and CHP-212 cells) were treated with or without poly-I:C (10 µg/ml; A–C) or ssRNA (10 µg/ml; D) for 4 hr and 12 hr. Total cellular RNA extracted from poly-I:C- or ssRNA-treated or untreated NT2-N cells was subjected to real-time RT-PCR for IRF RNA quantification. The data are expressed as IRF RNA levels relative (-fold) to control (without treatment, which is defined as 1). The results are mean ± SD of triplicate cultures, representative of three experiments (⋆P < 0.05, ⋆⋆P < 0.01).