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. 2014 Feb 26;4:4186. doi: 10.1038/srep04186

Figure 1. Absence of ATM, BRCA1, MLH1 and XPC methylation in lung cancer cell lines and tumours.

Figure 1

DNA methylation in the promoter regions of the ATM, BRCA1, MLH1 and XPC genes was assessed in 11 lung cancer cell lines and 56 NSCLC tumours using MS-HRM. After bisulfite modification, methylated DNA that retains cytosines has a higher melting temperature compared with unmethylated DNA that contains thymine (uracil before PCR). All samples having different melting patterns compared with unmethylated DNA control (in blue) are considered as methylated. Promoter methylation in ATM, BRCA1, MLH1 and XPC was not detected in any of the lung cancer cell lines and the NSCLC tumours. The negative first derivative plot of three representative lung cancer cell line samples are shown for BRCA1 (Panel A), MLH1 (Panel B), ATM (Panel C) and XPC (Panel D). Absence of XPC methylation is seen for the two lung cancer cell lines (H1355 and Calu-1) that were previously reported to be methylated.