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. 2014 Feb 26;5:62. doi: 10.3389/fpls.2014.00062

Figure 2.

Figure 2

The N-terminal region is required for the DcNMCP1 to localize to the nuclear periphery. Identification of the region required for localization to the nuclear periphery (A–D). Constructs of fusions are schematically represented in the left of micrographs. The micrographs show EGFP-fluorescence of A. graveolens cells expressing fusion proteins, and the fluorescence intensity distribution along the lines indicated in (B) and (D) are represented in (E) and (F). The fluorescence intensity was analyzed using ImageJ 1.46r software (http://rsbweb.nih.gov/ij/index.html). (G) Sequence similarity of the N-terminal regions between NMCP1 and NMCP2. (H) Effect of replacement of the first 141 amino acids in HR1-141T975 - 1053-E by the amino acids 1–150 region of DcNMCP2 on localization to the nuclear periphery. Bars = 5 μm.