Figure 2. RpaA is required for control of global gene expression by the PTO.
(Left) Gene expression timecourse upon rescue of Kai oscillator function in a ΔkaiBC background via ectopic expression of kaiBC (ΔkaiBC Ptrc::kaiBC, termed the “control clock rescue”). The heatmap shows the expression timecourse of the 471 genes that oscillate with a circadian period in the control clock rescue in each of two biological replicates, with genes normalized individually and ordered by phase. KaiC phosphorylation levels during the timecourse are shown below the heatmap.
(Right) Gene expression timecourse upon rescue of Kai oscillator function in the absence of rpaA via ectopic expression of kaiBC (ΔrpaA ΔkaiBC Ptrc::kaiBC, termed the “ΔrpaA clock rescue”). The heatmap shows the expression timecourse of the same set of genes as in (A), displayed in the same order. KaiC phosphorylation levels during the timecourse are shown below the heatmap.