Figure 5.

Purine–evoked Ca²⁺ waves between EG are lost with aging. (A) Representative Ca²⁺ response evoked by ADP in 9 EG (gray traces, average in green) within a myenteric ganglion from a 12 m.o.a. mouse. (B) Quantification of the number of neurons (blue) or glia (green) responding per myenteric ganglion to ADP in 2 (light shaded bars) and 12 m.o.a. mice (dark shaded bars; n = 6, mean ± SEM; ****P<.0001 between 2 and 12 m.o.a. glia, P=.1018 between 2 and 12 m.o.a. neurons, two-tailed t test). (C) Quantification of peak Ca²⁺ responses initiated by ADP in neurons (blue) or glia (green) in 2 (light shaded bars) and 12 (dark shaded bars) m.o.a. mice (n = 6, mean ± SEM; *P=.011 between 2 and 12 m.o.a. glia, *P=.0352 between 2 and 12 m.o.a. neurons, two-tailed t test).