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. Author manuscript; available in PMC: 2014 Feb 26.
Published in final edited form as: Nature. 2011 Mar 3;471(7336):74–79. doi: 10.1038/nature09803

Figure 4. Sae2 in VPA-treated cells.

Figure 4

a, 4HA–Sae2 was immunoprecipitated ± VPA with anti-HA and subsequently ± anti-acetyl-Lysine. Eluate was analysed using anti-HA. Lane 1: input Sae2; 2: input Sae2–HA –VPA; 3: as in 2 but + VPA; 4: 3 μl elution Sae2–HA – VPA after anti-HA immunoprecipitation (IP; input AcK-IP); 5: double amount of 4; 6: IP anti-AcK elution from anti-HA IP of Sae2–HA – VPA; 7: as in 6 but + VPA. b, SAE2::PK erg6Δ cells were treated as in Fig. 2a. After 30 min induction, VPA and PMSF were added or not. Samples were processed for western blot using anti-PK. c, Wild-type SAE2::PK, SAE2::PK atg1Δ and SAE2::PK atg19Δ cells were grown as in b. After 30 min induction, VPA was added or not and samples treated as in b. d, Wild-type SAE2::PK cells were grown as in b. After 120 min induction, rapamycin (200 ng ml−1) was added or not and samples were treated as in b.