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. Author manuscript; available in PMC: 2014 Feb 26.
Published in final edited form as: Kidney Int. 2010 Jul 14;78(7):668–678. doi: 10.1038/ki.2010.214

Figure 4. Serum- and glucocorticoid-regulated kinase 1 (SGK1) knockout (KO) suppressed mechanical stretch- or transforming growth factor (TGF)-β-induced epithelial–mesenchymal transition (EMT) in vitro.

Figure 4

(a, b) SGK1 KO inhibited mechanical stretch-induced cell morphology and cytoskeleton rearrangement. Kidney tubular cells from wild-type (WT) and SGK1 KO mice were treated with mechanical stretch for 24 h, and the morphology (a) and F-actin staining (b) were detected. (c) TGF-β-induced E-cadherin downregulation was inhibited by SGK1 KO. (d) Reexpression of SGK1 in SGK1 KO cells rescued the mechanical stretch- or TGF-β-caused E-cadherin downregulation. The expression of SGK1 in SGK1 KO cells was achieved by infection of SGK1 expression adenovirus. The lower panel shows the densitometry analysis. The data represent three independent experiments. *P<0.05 compared with control. **P<0.01 compared with control.