. Author manuscript; available in PMC: 2015 Feb 15.

Published in final edited form as: Biochem Pharmacol. 2013 Dec 16;87(4):547–561. doi: 10.1016/j.bcp.2013.11.020

Table 3.

PCR conditions.

Steps		Temperature (°C)			Duration (min)			Cycle number
Initial		94	94	94	6	4	4	1×	1×	1×
denaturation
	Denaturation	94	94	94	1	0.5	1
Amplification	Annealing	58	68	53	0.5	0.5	1	40×	38×	35×
	Extension	72	72	72	1	1	1
Final extension		72	72	72	5	5	10	1×	1×	1×
Hold		4	4	4	∞	∞	∞	1×	1×	1×

The conditions were for β2-AR (black font), GPR55 (red font), and GAPDH (green font).