Figure 3.

Multiple mouse antibodies demonstrate positive staining with a biotin-free mouse-on-mouse method. The primary antibody was combined with the Fab fragment of a rabbit anti-mouse antibody and detected using an anti-rabbit polymer. The images show staining for anti-muscle actin (HHF35) (A), anti-β−catenin (B), anti-estrogen receptor (C), anti-desmin (D), anti-aspergillus (E), anti-SV-40 (F), anti-smooth muscle actin (SMA) (G), anti-hepatocyte (H), anti-cytokeratin (AE1/AE3) (I). Images were captured at 40x. Scale bar is 50 µm.