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. 2014 Mar;62(3):172–184. doi: 10.1369/0022155413517701

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© The Author(s) 2014

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page(http://www.uk.sagepub.com/aboutus/openaccess.htm).

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Figure 2. — PKCα localizes to focal adhesions. Endogenous PKCα was detected in rat embryonic fibroblasts by indirect immunofluorescence microscopy. Cells were fixed in 4% paraformaldehyde containing 0.1% Tween 20 in phosphate-buffered saline followed by sequential incubations with anti-PKCα (clone M4, Millipore, Billerica, MA) and Alexa488-labelled anti mouse IgG (green; Molecular Probes, Carlsbad, CA). Filamentous actin (F-actin) was visualized using Phalloidin-Alexa568 (red; Molecular Probes). Images were captured on a Zeiss Axioplan epifluorescence microscope, and intensity and contrast were enhanced using Adobe Photoshop CS6 (Adobe Systems Inc., San Jose, CA). Arrows show co-localization of PKCα with termini of actin stress fibers. Bar = 10 μm.