Fig. 4. Analysis of baculovirus expressed chimeric L1+69-81 and L1+108-120 proteins by ELISA.
BPV1 wild type L1 (triangles), and chimeric L1+108-120 (squares) or L1+69-81 (diamonds) proteins were analyzed under native conditions by ELISA, using the indicated serial dilutions of mAb 5B6 directed to a conformation-dependent neutralizing epitope (A), or mAb AU-1 directed to a linear BPV1 L1 epitope under denaturing conditions (B). OD, absorbance at 405 nm, mean + S.D. of triplicate wells.