Fig. 6.

PPI maps between SlTPLs and SlIAAs established by a Y2H screen. (A) Yeast growth of co-transformed BD–TPLs and AD–IAAs. The yeast clones grown on selected medium lacking Trp, Leu, His, and Ade (TLHA) were scratched again on a TLHA plate. After 3–4 d, the growth of the yeast strains confirmed a positive interaction, as shown. AD–empty vector and AD–T7 vector were used as negative controls. (B) Schematic representation of the interaction map between SlTPLs and SlIAAs. Green indicates that the yeast grew quickly, less than 4 or 5 d after co-transformation, indicating a strong interaction between the SlTPL and SlIAA partners. Yellow indicates that the yeast grew slowly 7–8 d after co-transformation, indicating a weak interaction between the tested SlTPL and SlIAA. Red indicates that there was no interaction detected between the tested SlTPLs and SlIAAs. (C) Truncated form of SlTPL1 protein lacking the N-terminal LisH domain N-terminal used as a negative control. (This figure is available in colour at JXB online.)