Fig. 5.

Transcript and protein analysis from leaves of sg1 plants at different stages. (A–D) The expression levels of plastid-encoded polymerase (PEP) transcribed chloroplast genes, nucleus-encoded polymerase (NEP) transcribed chloroplast genes, nuclear-encoded chloroplast genes, and chlorophyll biosynthesis genes. (E) Total protein resolved by SDS–PAGE from leaves of sg1 and corresponding wild-type plants at different growth stages. The terms sg1-I, sg1-II, and sg1-III refer to the sixth leaves that exhibited albino, pale-green, and green leaves in 3- to 5-week-old sg1 mutants, respectively; Col-I, Col-II, and Col-III refer to the corresponding leaves in Col plants. Western blotting of β-tubulin was used to confirm equal loading.