Fig. 9.

APUM23 functions in 18S rRNA biosynthesis. (A) Colonies of the haploid nop9 mutant transformed with empty vector BG1805, NOP9, and APUM23, as indicated. (B) Comparisons of a dilution series of haploid nop9 mutant cells transformed with NOP9 (upper) and APUM23 (lower), plated on Magic Medium. Cells with APUM23 form smaller colonies than those with NOP9. Fold-dilution is indicated above each column. (C) Enrichment of unprocessed 35S pre-RNA in apum23-3 is revealed by semiquantitative reverse-transcription PCR. Error bar represents the standard error of the mean. UBC1 serves as the loading control.