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. 2014 Jan 24;65(4):1193–1203. doi: 10.1093/jxb/ert482

Fig. 4.

Fig. 4.

FUL over-expression suppresses the effects of high levels of FLC. (A) Vernalization response of FRI;FLC and FRI;FLC ful-2 in LD. The ful-2 mutation greatly enhances the late flowering phenotype of FRI;FLC unvernalized plants (left), while a vernalization treatment causes both genotypes to flower similarly earlier (right). (B–G) Histochemical detection of FLC::GUS activity in FRI;FLC (B–D) and FRI;FLC 35S::FUL (E–G) plants. Apices of 10-d-old plants are compared in (B) and (E), the first rosette leaf in (C) and (F), and inflorescence apices of plants at bolting in (D) and (G). All plants were heterozygous for the FLC::GUS reporter and for the wild-type dominant alleles of FRI or FLC. 35S::FUL in (E–G) was also heterozygous. Scale bars: 500 µm (B, C, E, F) or 100 µm (D, G). (H) Relative expression of FLC analysed by qRT-PCR in FRI;FLC and FRI;FLC 35S::FUL plants 10 d after germination. The error bars depict the s.e. based on two biological replicates. An asterisk (*) indicates a significant difference (P <0.05) from the WT control according to Student’s t-test.