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. 2014 Jan 21;65(4):1205–1215. doi: 10.1093/jxb/ert484

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© The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 6. — FLS2 and its signalling partners participate in regulating AtCLCd gene expression. (A) Expression of AtCLCd in Col-0 and fls2 mutant leaves analysed by semi-quantitative PCR. The level of ACTIN7 transcript was used as a loading control. (B–D) Expression of AtCLCd determined by quantitative RT–PCR in Col-0, Ws-0, and fls2 plants (B), in Col-0 and an FLS2-overexpressing line (OE-FLS2) (C), and in Col-0, bak1-5, bkk1-1, and bak1-5/bkk1-1 plants (D). (E and F) Expression levels of AtCLCd in Col-0, Ws-0, fls2, OE-FLS2 (E); and bak1-5, bkk1-1, and bak1-5/bkk1-1 (F) 14-day-old seedlings grown under sterile conditions. All samples were assayed in triplicate and normalized to ACTIN2. Error bars indicate the SD.