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. 2013 Nov 21;25(3):547–563. doi: 10.1681/ASN.2012101014

Figure 6.

Figure 6.

Renal epithelial-specific deletion of the Adora2b has no impact on the severity of diabetic nephropathy. Mice with deletion of the Adora2b in tubular epithelia of the kidneys were generated using the Cre-flox system (Adora2bloxP/loxP PEPCK-Cre+). Adora2bloxP/loxP PEPCK-Cre+ mice and age-, weight-, and sex-matched PEPCK-Cre+ mice were subjected to STZ-induced diabetes for 16 weeks before blood, urine, and organs were taken for measurement of (A) albumin excretion, (B) renal nephrin transcript, (C) renal VEGF transcript, and (D) urine MCP-1 (n=6–8 in each group). (E) Extracellular matrix deposition as determined by PAS staining. PAS staining was increased in diabetic PEPCK-Cre+ mice and in Adora2bloxP/loxP PEPCK-Cre+ mice (arrows; original magnification, ×400). (F) Histologic score of PAS staining sections. (G) Glomerular size in all groups. (H) Mesangial expansion. G and H show the result of 30 glomeruli in sections of six mice per group. DM, diabetes mellitus; n.s., not significant. All data are shown as mean±SD.