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Assays for autoantibodies specific for LAMP-2. Sera from ANCA-negative patients bind to hLAMP-2 using recombinant, E. coli expressed GST fusion protein by ELISA (A) and Western blot (B). (C) Reactivity is confirmed by indirect immunofluorescence on ldlD parental cells (ldlD) or cells expressing hLAMP-2 on the cell surface (ldlD/hLAMP-2H).
