Figure 1.

Vitamin D suppresses expression of hepcidin (HAMP) in human monocytes and hepatocytes. Effect of in vitro treatment of PBMC monocytes (PBMCm), monocytic THP1 cells, and HepG2 hepatocytic cells with vehicle, 25D (100 nM), or 1,25D (5 nM) for 6 hours on expression of mRNA for HAMP (A), CAMP (B), and CYP24A1 (C). Data are shown as mean fold-change in gene expression (±SD) relative to vehicle (0.1% ethanol) controls. *P<0.05; **P<0.01; ***P<0.001, statistically different from vehicle-treated cells. For RT-PCR data, n=8 separate donors for PBMC monocytes, n=4 separate cultures of THP1 cells, and n=5 separate cultures of HepG2 cells.