Figure 3.

The effect of lithium on different cell cycle markers. (A and C) mpkCCD cells are cultured and treated with lithium on Transwell filters (A) or in matrigel (C) as described in the legend for Figure 1. After three days, cells are lysed and immunoblotted for PCNA, cyclin D1, Histone H3, pHistone-H3, and β-actin. (B and D) Quantification of protein abundances of filter (A) or matrigel (C) grown cells, corrected for β-actin and related to the abundances of control cells of day 4 (n=4 for each condition and time point). *P<0.05, significant difference from control (CTR).