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. 2014 Jan 9;25(3):501–510. doi: 10.1681/ASN.2013090988

Figure 4.

Figure 4.

Lithium treatment of mpkCCD cells causes accumulation in the G2 phase. mpkCCD cells are cultured and treated with lithium on Transwell filters (A–C) or in matrigel (D–F) as described in the legend for Figure 1 and the percentage of cells in the G2 phase is determined (Transwell: n=6 for each condition and time point; matrigel: n=4 for each condition). Cell lysates of Transwell filter-cultured (B) or matrigel-cultured (D) cells are lysed and immunoblotted for cyclin B1, CDK1, pCDK1, and β-actin. (C and F) Quantification of protein abundances of filter (B) or matrigel (E) grown cells, corrected for β-actin and related to the abundances of control cells of day 4 (n=4 for each condition and time point). *P<0.05, significant difference from control (CTR).