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Effects of hypoxia on the differential regulation of nCLU expression and cell viability in PC3M and LNCaP cells. PC3M and LNCaP cells were exposed to 1% O2 for 6, 9, and 24 h. Expression of HIF-1α and nCLU were analyzed by real-time PCR (A) and Western blotting (B). (C) Cell viability was measured by MTT assay after exposure of cells to 1% O2 for 24 h. ***P < 0.001, **P < 0.005, *P < 0.01, compared to controls.
