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. Author manuscript; available in PMC: 2014 Feb 26.
Published in final edited form as: Small Rumin Res. 2013 Apr 30;113(2-3):411–420. doi: 10.1016/j.smallrumres.2013.03.014

Table 1.

Library of goat milk oligosaccharides described in the literature and validated by MS/MS in the present study. Compositions (Comp.) are indicated as total residue numbers (RN) of hexose, fucose, Nacetylhexosamine, N-acetylneuraminic acid, and N-glycolylneuraminic acid.

Comp. RN Neutral (N) or acidic (A) OS? Neutral mass (Da) MS/MS Conc. reported (g/L) Name (abbreviation) Structure Ref.a
21000 N 488.174 ✓ n.rb α-2′-Fucosyl-lactose (2′-FL) α-l-Fuc.p-(1-2)-β-d-Gal.p-(1-4)-d-Glc 1
30000 N 504.169 ✓ 0.03–0.05 α-3′-Galactosyl-lactose (3-GL) α-d-Gal.p-(1-3)-β-d-Gal.p-(1-4)-d-Glc 1-3
30000 N 504.169 ✓ n.rb β-6′-Galactosyl-lactose (6-GL) β-d-Gal.p-(1-6)-β-d-Gal.p-(1-4)-d-Glc 1, 2
11100c N 529.201 ✓ Fucosyl-lactosamine
20100 N 545.196 ✓ 0.02–0.04 6′-N-Acetyl-glucosaminyl-lactose (NAL) β-d-GlcNAc-(1-6)-β-d-Gal.p-(1-4)-d-Glc 2-4
20010 A 633.212 ✓ 0.05–0.07 6′-Sialyl-lactose (6-SL) Neu5Ac-(α2-6)-Gal-(β1-4)-Glc 2, 3, 5
20010 A 633.212 ✓ 0.03–0.05 3′-Sialyl-lactose (3-SL) Neu5Ac-(α2-3)-Gal-(β1-4)-Glc 2, 3, 5
20001 A 649.206 ✓ 0.04–0.06 6′-Glycolyl-neuraminyl-lactose (NGL) Neu5Gc-(α2-6)-Gal-(β1-4)-Glc 2, 3, 5
40000c N 666.222 ✓
10110 A 674.238 ✓ n.rb 6′-Sialyl-lactosamine Neu5Ac-(α2-6)-Gal-(β1-4)-GlcNAc 3, 5
10101c A 690.233 ✓ Glycolyl-neuraminyl-lactosamine
30100 N 707.248 ✓ Trace N-Acetyl-glucosaminyl-hexosyl-lactose (NAHL) Gal-(β1-4)-GIcNAc-(β1-6)-Gal-(1-4)-Glc 2, 4
20200 N 748.275 ✓ Trace Di-N-acetyl-glucosaminyl-lactose (DNAL) 2
30010 A 795.264 ✓ Trace 3′-Sialyl-6′galactosyl-lactose (3-SHL) graphic file with name nihms547794t1.jpg 2, 6
30010 A 795.264 ✓ Trace 6′-Sialyl-3′galactosyl-lactose (6-SHL) graphic file with name nihms547794t2.jpg 2, 5, 6
30001 A 811.259 ✓ Trace N-Glycolyl-neuraminyl-hexosyl-lactose (SNGHL) 2
50000c N 828.275 ✓
20110c A 836.291 ✓ Sialyl-N-acetylglucosaminyl-lactose (SNAL)
31100 N 853.306 ✓ n.rb Lacto-N-fuco-pentaose III (LNFP III) graphic file with name nihms547794t3.jpg 7
31100 N 853.306 ✓ n.rb Lacto-N-fuco-pentaose V (LNFP III) graphic file with name nihms547794t4.jpg 7
40100 N 869.301 ✓ Trace N-Acetyl-glucosaminyl-dihexosyl-lactose (NADHL) graphic file with name nihms547794t5.jpg 2, 4
30200c N 910.328 ✓
20020 A 924.307 ✓ 0.001–0.005 Disialyl-lactose (DSL) Neu5Ac-(α2-8)-Neu5Ac-(α2-3)-Gal-(β1-4)-Glc 2, 3
20011 A 940.302 Trace Sialyl-N-glycolyl-neuraminyl-lactose (SNGL) 2
20002 A 956.297 Trace Di-N-glycolyl-neuraminyl-lactose (DNGL) 2
40010 A 957.317 Trace Sialyl-di-hexosyl-lactose (SDHL) 2
6000c N 990.327 ✓
40200 N 1072.381 ✓ 0.001–0.005 Lacto-N-hexaose (LNH) graphic file with name nihms547794t6.jpg 2
30020 A 1086.360 Trace Disialyl-hexosyl-lactose 2
30011 A 1102.355 Trace Sialyl-N-glycolyl-neuraminyl-hexosyl-lactose (SNGHL) 2
30300c N 1113.407 ✓
30002 A 1118.350 Trace Di-N-glycolyl-neuraminyl-hexosyl-lactose (DNGHL) 2
20400c N 1154.434 ✓
41200c N 1218.438 ✓
31300c N 1259.465 ✓
40300 N 1275.460 Trace N-Acetyl-glucosaminyl-lacto-N-hexaose (NALNH) 2
40210 A 1363.476 Trace Sialyl-lacto-N-hexaose (SNLH) 2
a

References numbers corresponds to the following literature citations: (1) Urashima et al. (1994); (2) Martinez-Ferez et al. (2006a,b); (3) Mehra and Kelly (2006); (4) Chaturvedi and Sharma (1988); (5) Urashima et al. (1997); (6) Viverge et al. (1997); and (7) Chaturvedi and Sharma (1990a,b).

b

Not reported.

c

Newly identified OS.