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. Author manuscript; available in PMC: 2014 Feb 26.
Published in final edited form as: J Neurosci Methods. 2013 Oct 1;221:230–232. doi: 10.1016/j.jneumeth.2013.09.008

Fig. 1.

Fig. 1

WAAVES Program Flowchart. The order of separation criteria is an important factor when developing an automated analysis program such as WAAVES. (1) Sound files (.wav) are read into the program. (2) Sound objects are identified within the files for closer inspection. (3) Sound objects spanning 5 kHz and above consecutive frequencies in 0.5 ms are considered noise. (4) Within the experimental apparatus, noise generated by reverberations from high decibel 22–28 kHz USVs often appears at 40 and 60 kHz. (5) Sound objects defined as noise if frequency range > 60 kHz and mean frequency > 90 kHz. (6) Sound objects defined as noise if <2.5 ms. (7) Inter-call interval at least 10 ms for call count > 1. (8) Call duration setting (e.g., 5 or 20 ms). (9) Median value of change in mean frequency in 0.5 ms steps was required to be >0 and <2 kHz. (10) At this point calls have been successfully separated from noise and further USV subcategories can be created (e.g. flat versus frequency-modulated (FM)).