Skip to main content
. Author manuscript; available in PMC: 2014 Feb 26.
Published in final edited form as: Methods Mol Biol. 2013;1077:203–215. doi: 10.1007/978-1-62703-637-5_14

Figure 4. Q1 ion scanning of the NAD+ fraction isolated by HPLC.

Figure 4

Liquid chromatography-tandem mass spectrometry (LC/MS/MS) was performed with a Shimadzu 10A HPLC system coupled to a TSQ Quantum Ultra triple quadrupole mass spectrometer in the positive mode. The NAD+ peak was fractionated from a regular chow-fed liver extract by HPLC with a C18 column and analyzed by LC/MS/MS. NAD+ standard (Molecular weight=663.4) and blank (buffer) samples were also analyzed. Total ion chromatograms and Q1 scan spectra of indicated peaks are shown.