Table 2. Fitted kinetic parameters for β-hematin formation mediated by commercial phospholipids and biological lipids from R. prolixus midgut.

Lipid	% overall conversion	% contribution to overall reaction	n	Rate constant (z)	Half-life (minutes)
uPC (early)	38±3	44±3	1	1.0±0.3 min−1	0.7±0.2
uPC (late)	88±2	66±3	4	2.7±0.3×10−11 min−4	402±12
uPS	68.8±0.8a	100	2	0.00002±0.00001 min−2	225±10
uPE	62±1a,c	71± 2	1	17.1±5.1 min−1	0.04±0.01*
Blend	39.8±3.8a,d,e	54±5	1	19.7±16.6 min−1	0.035±0.030*
RML plasma	77.0±6.5b,f,g	72±5	1	0.039±0.003 min−1	17.7±1.4
RML blood	71.5±1.6b,f,g	71± 3	1	0.070±0.008 min−1	9.9±1.1

Values were expressed as mean ± SEM of five distinct kinetic parameters obtained from the data shown in Figure 4. Statistical analyses between groups were performed by using the Mann-Whitney (superscript letters) or Student's t tests (superscript symbols). ^ap<0.05 relative to uPC; ^bp<0.01 relative to uPC; ^cp<0.05 relative to uPS; ^dp<0.005 relative to uPS; ^ep<0.005 relative to uPE; ^fp<0.001 relative to uPE; ^gp<0.0005 relative to RML plasma. Comparisons of reactions half-lives showed that all groups were statistically distinct among each other (p<0.03), with exception of the uPE and Blend comparison (p = 0.88).