Table 1. Crystallographic statistics.
| Complex | CFP-10/ESAT-6 | sagEsxA | sagEsxA | sagEsxA |
| (3FAV) | SeMet | native I (3GVM) | native II (3GWK) | |
| Wavelength (Å) | 1.033 | 0.979 | 1.000 | 0.900 |
| Space group | C2 | P21212 | P21212 | P6522 |
| a, b, c (Å) | 160.34, 23.93, 83.86 | 132.44, 132.52, 43.49 | 133.10, 132.53, 43.67 | 76.58, 76.58, 149.74 |
| β (°) | 94.36 | |||
| Resolution range (Å)a | 20-2.15 | 50.0-2.50 | 50-2.10 | 50-1.30 |
| (2.21-2.15) | (2.57-2.50) | (2.21-2.10) | (1.33-1.30) | |
| Measured reflections | 58053 | 138669 | 263051 | 678917 |
| Unique reflectionsa | 17336 (978) | 50045 (3650) | 43011 (3128) | 62862 (4438) |
| R sym b(%)a | 5.5 (19.7) | 4.0 (13.1) | 9.1 (50.7) | 5.3 (47) |
| R meas c(%)a | 6.5 (26.1) | 4.9 (16.0) | 10 (55.4) | 5.5 (49.9) |
| Completeness | 95.5 (73.4) | 98 (94.7) | 99.9 (99.9) | 97.6 (95.2) |
| <I/σ(I)> g | 14.4 (4.2) | 19.8 (7.8) | 16.8 (3.8) | 23 (5.1) |
| Multiplicitya | 3.3 (2.2) | 2.8 (2.7) | 6.1 (6.1) | 10.8 (7.5) |
| Average B (Å2), overall/main chain | 33.9/32.1 | 32.1/30.3 | 20.7/18.3 | |
| R cryst d(%) | 20.1 | 18.5 | 14.8 | |
| R free e(%) | 23.3 | 21.5 | 18.8 | |
| RMSD from ideal | ||||
| Bond length (Å) | 0.023 | 0.008 | 0.017 | |
| Bond angles (°) | 1.34 | 0.89 | 1.32 | |
| Dihedral angles (°) | 17.8 | 19.0 | 17.4 | |
| Ramachandran plotf | ||||
| Most favoured region (%) | 99.3 | 98.6 | 98.4 | |
| Additional allowed regions (%) | 0.7 | 1.4 | 1.6 |
Numbers given in brackets are from the last resolution shell.
Rsym = (ΣhklΣi|Ii(hkl)-<I(hkl)>)/ΣhklΣIi(hkl), where Ii(hkl) is the intensity of the ith measurement of reflection (hkl) and <I(hkl)> is the average intensity.
Rmeas = (Σhkl (sqrt(Nhkl/(Nhkl-1))Σi|Ii(hkl)-<I(hkl)>)/ΣhklΣIi(hkl), where Ii(hkl) is the intensity of the ith measurement of reflection (hkl) and <I(hkl)> is the average intensity.
Rwork = (Σhkl|Fo-Fc|/ΣhklFo where Fo and Fc are the observed and calculated structure factors.
Rfree is calculated as for Rwork but from a randomly selected subset of the data (5%) which were excluded from the refinement [48].
Ramachandran et al., 1963 [49].
I is the integrated intensity and σ(I) is the estimated standard deviation of that intensity.