Table 2. Sequences of primers used to quantify mRNA by PCR (Q-PCR analysis) alongside the melting temperature (Tm) of each primer set.

Gene	Sequence 5′ to 3′	Length (bp)	% GC content	Tm (°C)	Product length (bp)
β-Actin forward	ATGCTCCCCGGGCTGTAT	18	61	60.5	63
β-Actin reverse	CATAGGAGTCCTTCTGACCCATTC	24	50	60.2
Slc7a5 forward	CTGGTCTTCGCCACCTACTT	20	55	59.4	127
Slc7a5 reverse	GCCTTTACGCTGTAGCAGTTC	21	52	59.6
Slc7a8 forward	AAGAAGCCTGACATTCCCCG	20	55	60.0	180
Slc7a8 reverse	TGTGTTGCCAGTAGACACCC	20	55	59.9
ATF-4 forward	AGCAAAACAAGACAGCAGCC	20	50	59.6	192
ATF-4 reverse	ACTCTCTTCTTCCCCCTTGC	20	55	59.0