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. 2014 Feb 26;9(2):e89834. doi: 10.1371/journal.pone.0089834

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© 2014 Beaver et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Formation of DU145 colonies from single cells was tracked by time lapse photography (Incucyte) every 4 hours from initial adherence for two weeks. (B) The number of colonies originating from 1 or more cells was determined. Colonies which were derived from a single cell upon initial adherence, but merged with other colonies were also determined. Results are displayed as mean ± S.E. from 5 experiments tracking 40 cells per experiment.