Figure 1. Vessel fractions are enriched in vascular-associated proteins.

S1 fractions were enriched in proteins associated with various cellular compartments of cerebral vessels as demonstrated by increased levels of Occludin, PECAM, SMA and GFAP. α-tubulin showed similar expression across all blots. (A–D) Levels of selected proteins (relative to respective α-tubulin levels) were all significantly enriched in the S1 fraction compared to whole brain samples (n = 3/gp; p<0.001; t-test). WB = whole brain fraction; S1 = vessel-enriched fraction.