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. 2014 Feb 26;9(2):e89970. doi: 10.1371/journal.pone.0089970

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© 2014 Searcy et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Tissue sections representative of independent 3 and 9 month-old WT (A&B) and 3 and 9 month-old Tg-SwDI (C&D) animals were triple-labelled with HTRA1 (red), 6E10 (green) and β-dystroglycan (magenta) and analysed with a laser scanning confocal microscope (400×). Immunohistochemical analysis of the four different cohorts show HTRA1 closely associated with Aβ-laden vessels [white arrow, (D)] within the thalamus of 9 month-old Tg-SwDI mice (E–J) and appeared extracellular.