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. 2014 Feb 26;9(2):e89968. doi: 10.1371/journal.pone.0089968

Figure 2. Assessment of competitive PS- or apoptosis-binding of apoptosis-targeting PEG-b-PCL micelles carrying DiR by PS saturation with free peptides in vitro.

Figure 2

Results are presented as %FLI DiR molecules bound on plates and %FLI from DiR molecules bound on ES-2-luc ovarian tumor spheroids. BLI from ES-2-luc cells and FLI from DiR molecules were quantified using Xenogen IVIS 200 Series. (A) Competitive binding test of apoptosis-targeting PEG-b-PCL micelles carrying DiR (1.0 µM peptide and 500 nM DiR) to PC- or PS-coated 96-well plates (total 200 µM of phospholipid). (B) Competitive binding test of apoptosis-targeting PEG-b-PCL micelles carrying DiR to apoptosis-induced ES-2-luc ovarian tumor spheroids (** <0.01, *** <0.001).