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. 2014 Feb 26;9(2):e90077. doi: 10.1371/journal.pone.0090077

Figure 3. Effect of Dmlc2 mutations on pulse song properties.

Figure 3

(A) Representative pulse song oscillograms from Control, Ext, Phos and Dual males (top to bottom panels, respectively). (B) Phos and Dual males sing with similar cycles per pulse (CPP) and (C) pulse length (PL) compared to Control males. Ext males produce songs with higher CPP and longer PL. (D) All the mutant males produce pulse song with normal intrapulse frequency (IPF), with only the Phos mutants’ IPF showing a slight reduction compared to Ext and Dual. (E) None of the mutations affect interpulse interval. (F) The Dual mutant has significantly reduced pulse duty cycle compared to Control, Ext, and Phos. (G) Amplitude ratio (AMP-RT) of consecutive sine to pulse song is significantly higher in individual (Ext, Phos) and Dual mutants compared to Control. n = 7–8 males for each line. *(p<0.05), ***(p<0.001), ****(p<0.0001) indicate significant differences from Control. § (p<0.05), Ω (p<0.05) and Δ (p<0.05) indicate significant difference from Ext, Phos and Dual mutants, respectively. Error bars indicate SEM.