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. Author manuscript; available in PMC: 2014 Jul 17.
Published in final edited form as: Nat Commun. 2014;5:3077. doi: 10.1038/ncomms4077

Figure 6. Structural determinants of DNA compaction.

Figure 6

(A) A schematic of the TPM assay. The bead is attached by a single DNA molecule to the glass surface. Upon addition of TFAM, the contour length of the DNA molecule is reduced, causing a decrease in the bead’s radius of motion. (B) Effect of increasing concentrations of TFAM on the DNA contour length. A 1910 bp DNA fragment was used. Error bars indicate standard error of the mean from three independent experiments. (C) DNA compaction by TFAM on DNA fragments of varying lengths. Error bars represent standard error of the mean from three independent experiments. (D) Fractional shortening of DNA by TFAM as a function of DNA length. (E) Maximal DNA compaction by wild-type TFAM and mutants. A 1910 bp DNA fragment was used. Error bars illustrate standard error of the mean from three independent experiments.