Figure 2.

Increased expression of CSPG in reactive astrocytes in the peri-infarct region of human ischemic brain. A. Double immunocytochemistry was performed on the ischemic brain sections using anti-GFAP (red) and anti-CSPG (green). The images were recorded using a 2-photon confocal microscope. Representative images show that CSPG was predominantly expressed in GFAP-positive reactive astrocytes. B. Higher magnification view of insert in 2A. C. Confocal images of double-label immunohistochemistry on the ischemic brain section using anti-GFAP (red) and anti-ED1 (green). D. Higher magnification shows GFAP-positive reactive astrocytes (red) not co-localizing with ED1-postive activated micrglia (green). DAPI (blue) was used for nuclei counterstains.