Table 1. Comparison of commercially available sclerostin assays.
| Assay source | Assay type | Primary antibody | Secondary antibody | Intra-assay CV (%) | Inter-assay CV (%) | Detection limit (pmol/l) | Detection range (pmol/l) |
|---|---|---|---|---|---|---|---|
| Biomedica | ELISA | Polyclonal | Monoclonal | 5 | 6 | 2.6 | 0–240 |
| TECO | ELISA | Polyclonal | Monoclonal | <10 | <10 | 5.7 | 11–176 |
| MSD | ECL | Polyclonal | Polyclonal | 6 | 10 | 0.1 | 1–440 |
Abbreviations: CV, coefficient of variation; ECL, electro-chemiluminescence assay; ELISA, enzyme-linked immunosorbent assay; MSD, Meso Scale Discovery; TECO, TECO Medical Group.