Table 1.
Primer sequence for amplification of A-amoA, B-amoA, nosZ and nirS gene from soil and construction of the plasmid standard
| Primers | Target | Sequence (5ʹ to 3ʹ) | Size of PCR amplicon (bp) |
|---|---|---|---|
| BA-F | amoA (AOB) | GGHGACTGGGAYTTCTGG | 696 |
| B-R | CGTCTAAGCCAGACCATTAStctagaCCTCKGSAAAGCCTTCTTC | ||
| A-F | amoA (AOA) | GAAGAAGGCTTTSCMGAGGtctagaSTAATGGTCTGGCTTAGACG | 689 |
| BA-R | CTGGCTGTCGAKGAACARSGWgcggccgcGCGGCCATCCATCTGTATGT | ||
| ZS-F | nosZ | ACATACAGATGGATGGCCGCgcggccgcWCSYTGTTCMTCGACAGCCAG | 313 |
| Z-R | CCSGTYTCCTTSACGTTSACggatccATGTCGATCARCTGVKCRTTYTC | ||
| S-F | nirS | GARAAYGMBCAGYTGATCGACATggatccGTSAACGTSAAGGARACSGG | 455 |
| ZS-R | GASTTCGGRTGSGTCTTGA |