Table 4.

Measured copy number concentration of digested and undigested plasmid obtained using ddPCR with three different restriction endonuclease enzyme digestions

	Digested plasmid copy number concentration (copies μL−1)a	Undigested plasmid copy number concentration (copies μL−1)a
BamH1	(4.69 ± 0.17) × 109*	(2.05 ± 0.08) × 109†
EcoR1	(4.77 ± 0.16) × 109*	(2.12 ± 0.06) × 109†
EcoR1 + XbaI	(4.70 ± 0.16) × 109*	(2.31 ± 0.05) × 109†

^aMean and standard deviation from five replicates

*,†The same symbol indicates no significant difference determined by t-test