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. 2013 Oct 13;98(6):2819–2828. doi: 10.1007/s00253-013-5287-x

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© The Author(s) 2013

Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.

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Fig. 2 — a SDS-PAGE analysis of Chi18H8 purification. Lanes 1 and 2, E. coli pGEX-6P-3::chi18H8 insoluble and soluble fractions; lane 3, elution with reduced glutathione of the GST-chi18H8 fusion protein; lane 4, Chi18H8 after on-column cleavage of GST-tag; lane 5, elution with reduced glutathione of the GST-tag and PreScission protease; lane 6, PreScission buffer for the on-column cleavage. LMW Marker (PageRuler™ Prestained Protein Ladder, Fermentas); lane S standard Rhodotorula gracilis His₆-d amino acid oxidase, 36 kDa, kindly provided by Prof. Pollegioni, University of Insubria. b Zymogram analysis of the purified GST-Chi18H8 (lane 1) and Chi18H8 (lane 2) with CM-chitin-RBV as substrate