Table 10.

Reverse mutagenicity assay results

Test	Chemical	Dose		Colonies/plate [factor]a)
Strain	Treated	(μg/plate)		With S9 mix	Without S9 mix
TA100	SEP	0		154 ± 3 [1.0]	143 ± 6 [1.0]
		15		139 ± 1 [0.9]	141 ± 8 [1.0]
		50		134 ± 10 [0.9]	152 ± 8 [1.1]
		150		122 ± 7 [0.8]	131 ± 11 [0.9]
		500		103 ± 4 [0.7]	139 ± 10 [1.0]
		1500	#	96 ± 6 [0.6]	109 ± 7 [0.8]
		5000	#	84 ± 6 [0.5]	91 ± 8 [0.6]
		0		12 ± 2 [1.0]	12 ± 3 [1.0]
		15		10 ± 2 [0.8]	13 ± 4 [1.1]
		50		12 ± 1 [1.1]	13 ± 3 [1.1]
TA1535	SEP	150		14 ± 2 [1.2]	12 ± 3 [1.0]
		500		9 ± 2 [0.8]	9 ± 3 [0.8]
		1500	#	8 ± 2 [0.7]	12 ± 4 [1.0]
		5000	#	11 ± 2 [0.9]	12 ± 1 [1.0]
		0		35 ± 3 [1.0]	23 ± 3 [1.0]
		15		25 ± 4 [0.7]	21 ± 4 [0.9]
		50		37 ± 3 [1.0]	30 ± 3 [1.3]
TA98	SEP	150		33 ± 1 [0.9]	21 ± 4 [0.9]
		500		25 ± 3 [0.7]	20 ± 3 [0.9]
		1500	#	29 ± 1 [0.8]	22 ± 3 [1.0]
		5000	#	33 ± 4 [1.0]	28 ± 5 [1.3]
		0		10 ± 1 [1.0]	9 ± 2 [1.0]
		15		9 ± 1 [0.9]	5 ± 2 [0.6]
		50		9 ± 2 [0.9]	6 ± 2 [0.7]
TA1537	SEP	150		9 ± 3 [0.9]	6 ± 3 [0.7]
		500		7 ± 3 [0.7]	8 ± 2 [0.9]
		1500	#	6 ± 1 [0.6]	5 ± 1 [0.6]
		5000	#	6 ± 1 [0.6]	6 ± 1 [0.6]
		0		20 ± 2 [1.0]	17 ± 1 [1.0]
		15		23 ± 2 [1.1]	15 ± 2 [0.9]
		50		22 ± 3 [1.1]	12 ± 2 [0.7]
E. coli	SEP	150		17 ± 2 [0.9]	18 ± 3 [1.1]
		500		20 ± 5 [1.0]	15 ± 2 [0.9]
		1500	#	18 ± 4 [0.9]	20 ± 4 [1.2]
		5000	#	26 ± 3 [1.3]	22 ± 4 [1.3]
Positive	controls
TA100	2-AA	1.0		728 ± 29 [4.7]
TA1535	2-AA	2.0		118 ± 8 [10.1]
TA98	B[a]P	1.0		272 ± 30 [7.8]
TA1537	2-AA	1.0		133 ± 21 [13.3]
E. coli	2-AA	6.0		225 ± 20 [11.1]
TA100	SA	0.5			464 ± 19 [3.3]
TA1535	SA	0.5			318 ± 18 [25.8]
TA98	2-NF	2.0			456 ± 56 [20.1]
TA1537	ICR-191	0.5			290 ± 35 [32.2]
E. coli	4NQO	0.5			177 ± 20 [10.6]

2-AA: 2-Aminoanthracene, B[a]P: Benzo[a]pyrene, Benzo[a]pyrene, SA: Sodium azide, 2-NF: 2-Nitrofluorene, 4NQO: 4-Nitroquinoline-1-oxide, ICR-191: Acridine Mutagen ICR 191.

#: Turbid in the treatment mixture.

^a)Three plates/dose were used. No. of colonies of treated plate/No. of colonies of negative control plate.