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. 2014 Feb 27;4:4217. doi: 10.1038/srep04217

Figure 2. HEK 293 cells efficiently secrete unglycosylated and N-glycosylated recombinant human CRISP3.

Figure 2

(A) Western blot with human CRISP3 antibody showing CRISP3 induction. (B) PNGase F treatment selectively affects the larger CRISP3 variant in both native (human seminal plasma, first two lanes) and recombinant (‘rCRISP3-His', last two lanes) protein. Full length gels are available under supplementary data.